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Authors
Affiliations
1 Doctoral Program of Medical Sciences, Brawijaya University, Malang, ID
2 Department of Pharmacology, Brawijaya University, Malang, ID
3 Department of Microbiology, Brawijaya University, Malang, ID
Source
Indian Journal of Public Health Research & Development, Vol 10, No 12 (2019), Pagination: 1770-1776
Abstract
Objective: To evaluate the bronchorelaxation effect of methanol extract of Coptosapelta flavescens Korth. ischolar_main (MECFR) on cholinergic, histamine receptors and beta2-adrenoceptors, in vitro.
Method: The research design was experimental in which a 4-mm long guinea pig’s bronchus was kept in an isolated organ bath containing Kreb’s-Henseleit solution at 37°C, pH 7.4 and flooded with carbogen gas. The study was divided into 3 groups: in Group 1 and 2, the bronchial rings were incubated in 3 concentrations of MECFR, and then contracted with cumulative doses of methacholine and histamine, respectively; in Group 3, the bronchial ring was incubated in 3 concentrations of propranolol, contracted with a single dose of methacholine, and then the MECFR was administered in cumulative doses.
Results: In both Group 1 and Group 2, the dose-response curves of all 3 doses of MECFR shifted to the right with lower Emax (P<0.05) as compared to the negative control. In Group 3, the dose-response curves showed MECFR has bronchorelaxation effect on bronchial rings both in the presence of propranolol andwithout, with similar Emax (P>0.05).
Conclusion: MECFR exhibits bronchorelaxation effects by acting as non-competitive antagonist at the cholinergic and histamine1 receptors, and also by acting as beta2-adrenoceptor agonist.
Keywords
Beta2-adrenoceptor, Cholinergicreceptor, Coptosapelta Flavescens Korth Root, Histamine Receptor.
